In 1605, when Anne was 14, the family moved from Alford to the heart of London, where her father was given the position of vicar of the Church of Saint Martin's in the Vintry.[11] Here his expression of puritan views, though somewhat muffled, were tolerated, because of a shortage of pastors.Marbury took on additional work in 1608, preaching in the parish of Saint Pancras, several miles northwest of the city, travelling there by horseback twice a week. In 1610 he replaced that position with one much closer to home, and became rector of Saint Margaret's, on New Fish Street, only a short walk from Saint Martin in the Vintry. He was at a high point in his career, but in February 1611, when Anne was 19 years old, he died suddenly at the age of 55.

Adulthood—following John Cotton]
The year after her father's death, Anne Marbury, aged 21, married William Hutchinson, a familiar acquaintance from Alford, who was a fabric merchant then working in London. The couple was married at St Mary Woolnoth Church in London on 9 August 1612, shortly after which they moved back to their hometown of Alford.[